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Black lipid membranes studied by x-ray phase contrast imaging

dc.contributor.authorBeerlink, André
dc.date.accessioned2011-05-31T09:09:21Z
dc.date.available2011-05-31T09:09:21Z
dc.date.issued2011
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/6361
dc.description.abstractMembranes fulfill many different functions inside a cell and have consequently been in the focus of biophysical studies for several decades. High resolution structure analysis of model membrane systems by x-ray reflectivity is typically performed using stabilizing solid supports, which have shown to strongly interact with the membranes and can severly affect the membrane`s functionality. To circumvent these restrictions free-standing lipid model membranes, also called Black Lipid Membranes (BLMs), have been used in this work towards a novel type of structural assay based on x-ray phase contrast imaging. Bulged lipid bilayers fully immersed in buffer solution have been visualized by a transmitted plane or spherical x-ray wave front in the Fresnel regime after free space propagation. The development of the experimental system and the image analysis enables a quantitative investigation of the membrane`s structural properties in a localized membrane patch. In future this technique can be used to study the structure of membranes and more generally ultrathin films in functional and physiological environments and under out-of-equilibrium conditions.
dc.format.extent1-140
dc.language.isoeng
dc.publisherUniversitätsverlag Göttingen
dc.identifier.bibliographicCitationGöttingen series in x-ray physics; 1
dc.rightsopenAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nd/2.0/de/
dc.subject.ddc530
dc.titleBlack lipid membranes studied by x-ray phase contrast imaging
dc.typemonograph
dc.subject.gokWCC 000
dc.subject.gokRQE 900
dc.subject.bk42.12
dc.subject.bk33.05
dc.publisher.placeGöttingen
dc.type.versionpublishedVersion
dc.type.subtypemonograph
dc.description.statuspeerReviewed


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