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Functional organization of a single nif cluster in the mesophilic archaeon Methanosarcina mazei strain Gö1

dc.contributor.authorEhlers, Claudia
dc.contributor.authorVeit, Katharina
dc.contributor.authorGottschalk, Gerhard
dc.contributor.authorSchmitz, Ruth A.
dc.date.accessioned2011-03-02T10:30:29Z
dc.date.available2011-03-02T10:30:29Z
dc.date.issued2002
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/5910
dc.description.abstractThe mesophilic methanogenic archaeon Methanosarcina mazei strain Gö1 is able to utilize molecular nitrogen (N2) as its sole nitrogen source. We have identified and characterized a single nitrogen fixation (nif) gene cluster in M. mazei Gö1 with an approximate length of 9 kbp. Sequence analysis revealed seven genes with sequence similarities to nifH, nifI1, nifI2, nifD, nifK, nifE and nifN, similar to other diazotrophic methanogens and certain bacteria such as Clostridium acetobutylicum, with the two glnB-like genes (nifI1 and nifI2) located between nifH and nifD. Phylogenetic analysis of deduced amino acid sequences for the nitrogenase structural genes of M. mazei Gö1 showed that they are most closely related to Methanosarcina barkeri nif2 genes, and also closely resemble those for the corresponding nif products of the gram-positive bacterium C. acetobutylicum. Northern blot analysis and reverse transcription PCR analysis demonstrated that the M. mazei nif genes constitute an operon transcribed only under nitrogen starvation as a single 8 kb transcript. Sequence analysis revealed a palindromic sequence at the transcriptional start site in front of the M. mazei nifH gene, which may have a function in transcriptional regulation of the nif operon.
dc.language.isoeng
dc.rightsopenAccess
dc.rights.urihttp://goedoc.uni-goettingen.de/goescholar/License_Goedoc_2009_03.txt
dc.subjectnif cluster
dc.titleFunctional organization of a single nif cluster in the mesophilic archaeon Methanosarcina mazei strain Gö1
dc.typejournalArticle
dc.identifier.doi10.1155/2002/362813
dc.type.versionpublishedVersion
dc.bibliographicCitation.volume1
dc.bibliographicCitation.issue2
dc.bibliographicCitation.firstPage143
dc.bibliographicCitation.lastPage150
dc.type.subtypejournalArticle
dc.description.statuspeerReviewed
dc.bibliographicCitation.journalArchaea


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