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Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity

dc.contributor.authorRuhlandt, Daja
dc.contributor.authorAndresen, Martin
dc.contributor.authorJensen, Nickels
dc.contributor.authorGregor, Ingo
dc.contributor.authorJakobs, Stefan
dc.contributor.authorEnderlein, Jörg
dc.contributor.authorChizhik, Alexey I.
dc.date.accessioned2021-05-17T14:49:01Z
dc.date.available2021-05-17T14:49:01Z
dc.date.issued2020de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/17780
dc.description.abstractOne of the key photophysical properties of fluorescent proteins that is most difficult to measure is the quantum yield. It describes how efficiently a fluorophore converts absorbed light into fluorescence. Its measurement using conventional methods become particularly problematic when it is unknown how many of the proposedly fluorescent molecules of a sample are indeed fluorescent (for example due to incomplete maturation, or the presence of photophysical dark states). Here, we use a plasmonic nanocavity-based method to measure absolute quantum yield values of commonly used fluorescent proteins. The method is calibration-free, does not require knowledge about maturation or potential dark states, and works on minute amounts of sample. The insensitivity of the nanocavity-based method to the presence of non-luminescent species allowed us to measure precisely the quantum yield of photo-switchable proteins in their on-state and to analyze the origin of the residual fluorescence of protein ensembles switched to the dark state.de
dc.description.sponsorshipOpen-Access-Publikationsfonds 2020
dc.language.isoengde
dc.rightsopenAccess
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.ddc530
dc.titleAbsolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavityde
dc.typejournalArticlede
dc.identifier.doi10.1038/s42003-020-01316-2
dc.type.versionpublishedVersionde
dc.relation.eISSN2399-3642
dc.bibliographicCitation.volume3de
dc.bibliographicCitation.issue1de
dc.type.subtypejournalArticle
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalCommunications Biologyde


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