dc.contributor.author | Ruhlandt, Daja | |
dc.contributor.author | Andresen, Martin | |
dc.contributor.author | Jensen, Nickels | |
dc.contributor.author | Gregor, Ingo | |
dc.contributor.author | Jakobs, Stefan | |
dc.contributor.author | Enderlein, Jörg | |
dc.contributor.author | Chizhik, Alexey I. | |
dc.date.accessioned | 2021-05-17T14:49:01Z | |
dc.date.available | 2021-05-17T14:49:01Z | |
dc.date.issued | 2020 | de |
dc.identifier.uri | http://resolver.sub.uni-goettingen.de/purl?gs-1/17780 | |
dc.description.abstract | One of the key photophysical properties of fluorescent proteins that is most difficult to measure is the quantum yield. It describes how efficiently a fluorophore converts absorbed light into fluorescence. Its measurement using conventional methods become particularly problematic when it is unknown how many of the proposedly fluorescent molecules of a sample are indeed fluorescent (for example due to incomplete maturation, or the presence of photophysical dark states). Here, we use a plasmonic nanocavity-based method to measure absolute quantum yield values of commonly used fluorescent proteins. The method is calibration-free, does not require knowledge about maturation or potential dark states, and works on minute amounts of sample. The insensitivity of the nanocavity-based method to the presence of non-luminescent species allowed us to measure precisely the quantum yield of photo-switchable proteins in their on-state and to analyze the origin of the residual fluorescence of protein ensembles switched to the dark state. | de |
dc.description.sponsorship | Open-Access-Publikationsfonds 2020 | |
dc.language.iso | eng | de |
dc.rights | openAccess | |
dc.rights | Namensnennung 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.ddc | 530 | |
dc.title | Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity | de |
dc.type | journalArticle | de |
dc.identifier.doi | 10.1038/s42003-020-01316-2 | |
dc.type.version | publishedVersion | de |
dc.relation.eISSN | 2399-3642 | |
dc.bibliographicCitation.volume | 3 | de |
dc.bibliographicCitation.issue | 1 | de |
dc.type.subtype | journalArticle | |
dc.description.status | peerReviewed | de |
dc.bibliographicCitation.journal | Communications Biology | de |