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Analyzing the Dietary Diary of Bumble Bee

dc.contributor.authorLeidenfrost, Robert M.
dc.contributor.authorBänsch, Svenja
dc.contributor.authorPrudnikow, Lisa
dc.contributor.authorBrenig, Bertram
dc.contributor.authorWestphal, Catrin
dc.contributor.authorWünschiers, Röbbe
dc.date.accessioned2020-06-09T13:26:22Z
dc.date.available2020-06-09T13:26:22Z
dc.date.issued2020de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/17385
dc.description.abstractBumble bees are important crop pollinators and provide important pollination services to their respective ecosystems. Their pollen diet and thus food preferences can be characterized through nucleic acid sequence analysis. We present ITS2 amplicon sequence data from pollen collected by bumble bees. The pollen was collected from six different bumble bee colonies that were placed in independent agricultural landscapes. We compared next-generation (Illumina), i.e., short-read, and thirdgeneration (Nanopore), i.e., MinION, sequencing techniques. MinION data were preprocessed using traditional and Nanopore specific tools for comparative analysis and were evaluated in comparison to short-read sequence data with conventional processing. Based on the results, the dietary diary of bumble bee in the studied landscapes can be identified. It is known that short reads generated by next-generation sequencers have the advantage of higher quality scores while Nanopore yields longer read lengths. We show that assignments to taxonomic units yield comparable results when querying against an ITS2-specific sequence database. Thus, lower sequence quality is compensated by longer read lengths. However, the Nanopore technology is improving in terms of data quality, much cheaper, and suitable for portable applications. With respect to the studied agricultural landscapes we found that bumble bees require higher plant diversity than only crops to fulfill their foraging requirements.de
dc.description.sponsorshipOpen-Access-Publikationsfonds 2020
dc.language.isoengde
dc.rightsopenAccess
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectbiodiversity; ecology; pollen; bumble bee; ITS2; next-generation sequencing; third-generation sequencing; Nanoporede
dc.subject.ddc630
dc.titleAnalyzing the Dietary Diary of Bumble Beede
dc.typejournalArticlede
dc.identifier.doi10.3389/fpls.2020.00287
dc.identifier.doi10.3389/fpls.2020.00287.s001
dc.type.versionpublishedVersionde
dc.relation.eISSN1664-462X
dc.bibliographicCitation.volume11de
dc.bibliographicCitation.firstPage1de
dc.bibliographicCitation.lastPage9de
dc.type.subtypejournalArticle
dc.bibliographicCitation.articlenumber287de
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalFrontiers in Plant Sciencede


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