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Transferability and Polymorphism of SSR Markers Located in Flavonoid Pathway Genes in Fragaria and Rubus Species

dc.contributor.authorLebedev, Vadim G.
dc.contributor.authorSubbotina, Natalya M.
dc.contributor.authorMaluchenko, Oleg P.
dc.contributor.authorLebedeva, Tatyana N.
dc.contributor.authorKrutovsky, Konstantin V.
dc.contributor.authorShestibratov, Konstantin A.
dc.date.accessioned2020-01-09T13:45:42Z
dc.date.available2020-01-09T13:45:42Z
dc.date.issued2020de
dc.relation.ISSN2073-4425de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/17052
dc.description.abstractStrawberry (Fragaria) and raspberry (Rubus) are very popular crops, and improving their nutritional quality and disease resistance are important tasks in their breeding programs that are becoming increasingly based on use of functional DNA markers. We identified 118 microsatellite (simple sequence repeat-SSR) loci in the nucleotide sequences of flavonoid biosynthesis and pathogenesis-related genes and developed 24 SSR markers representing some of these structural and regulatory genes. These markers were used to assess the genetic diversity of 48 Fragaria and Rubus specimens, including wild species and rare cultivars, which differ in berry color, ploidy, and origin. We have demonstrated that a high proportion of the developed markers are transferable within and between Fragaria and Rubus genera and are polymorphic. Transferability and polymorphism of the SSR markers depended on location of their polymerase chain reaction (PCR) primer annealing sites and microsatellite loci in genes, respectively. High polymorphism of the SSR markers in regulatory flavonoid biosynthesis genes suggests their allelic variability that can be potentially associated with differences in flavonoid accumulation and composition. This set of SSR markers may be a useful molecular tool in strawberry and raspberry breeding programs for improvement anthocyanin related traits.de
dc.description.sponsorshipOpen-Access-Publikationsfonds 2019
dc.language.isoengde
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectFragaria; Rubus; chitinase; exons; flavonoid biosynthesis pathway; introns; microsatellites; polymorphism; transcription factor genes; transferabilityde
dc.subject.ddc570
dc.titleTransferability and Polymorphism of SSR Markers Located in Flavonoid Pathway Genes in Fragaria and Rubus Speciesde
dc.typejournalArticlede
dc.identifier.doi10.3390/genes11010011
dc.type.versionpublishedVersionde
dc.relation.eISSN2073-4425
dc.bibliographicCitation.volume11de
dc.bibliographicCitation.issue1de
dc.bibliographicCitation.firstPage11de
dc.type.subtypejournalArticle
dc.identifier.pmid31877734
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalGenesde


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