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Mass spectrometry reveals the presence of specific set of epigenetic DNA modifications in the Norway spruce genome

dc.contributor.authorYakovlev, Igor A.
dc.contributor.authorGackowski, Daniel
dc.contributor.authorAbakir, Abdulkadir
dc.contributor.authorViejo, Marcos
dc.contributor.authorRuzov, Alexey
dc.contributor.authorOlinski, Ryszard
dc.contributor.authorStarczak, Marta
dc.contributor.authorFossdal, Carl Gunnar
dc.contributor.authorKrutovsky, Konstantin V.
dc.date.accessioned2020-01-09T13:29:33Z
dc.date.available2020-01-09T13:29:33Z
dc.date.issued2019de
dc.relation.ISSN2045-2322de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/17049
dc.description.abstract5-Methylcytosine (5mC) is an epigenetic modification involved in regulation of gene expression in metazoans and plants. Iron-(II)/α-ketoglutarate-dependent dioxygenases can oxidize 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Although these oxidized forms of 5mC may serve as demethylation intermediates or contribute to transcriptional regulation in animals and fungi, experimental evidence for their presence in plant genomes is ambiguous. Here, employing reversed-phase HPLC coupled with sensitive mass spectrometry, we demonstrated that, unlike 5caC, both 5hmC and 5fC are detectable in non-negligible quantities in the DNA of a conifer, Norway spruce. Remarkably, whereas 5hmC content of spruce DNA is approximately 100-fold lower relative to human colorectal carcinoma cells, the levels of both - 5fC and a thymine base modification, 5-hydroxymethyluracil, are comparable in these systems. We confirmed the presence of modified DNA bases by immunohistochemistry in Norway spruce buds based on peroxidase-conjugated antibodies and tyramide signal amplification. Our results reveal the presence of specific range of noncanonical DNA bases in conifer genomes implying potential roles for these modifications in plant development and homeostasis.de
dc.description.sponsorshipOpen-Access-Publikationsfonds 2019
dc.language.isoengde
dc.rightsopenAccess
dc.subjectMass spectrometry; epigenetic DNA; Norway spruce genomede
dc.subject.ddc570
dc.titleMass spectrometry reveals the presence of specific set of epigenetic DNA modifications in the Norway spruce genomede
dc.typejournalArticlede
dc.identifier.doi10.1038/s41598-019-55826-z
dc.type.versionpublishedVersionde
dc.relation.eISSN2045-2322
dc.bibliographicCitation.volume9de
dc.bibliographicCitation.issue1de
dc.type.subtypejournalArticle
dc.identifier.pmid31848418
dc.bibliographicCitation.articlenumber19314de
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalScientific Reportsde


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