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Subcellular Targeting of VIP Boutons in Mouse Barrel Cortex is Layer-Dependent and not Restricted to Interneurons

dc.contributor.authorZhou, Xiaojuan
dc.contributor.authorRickmann, Michael
dc.contributor.authorHafner, Georg
dc.contributor.authorStaiger, Jochen F.
dc.date.accessioned2019-12-09T12:23:28Z
dc.date.available2019-12-09T12:23:28Z
dc.date.issued2017de
dc.relation.ISSN1460-2199de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/16876
dc.description.abstractNeocortical vasoactive intestinal polypeptide (VIP) expressing cells are a diverse subpopulation of GABAergic interneurons issuing distinct axonal projections. They are known to inhibit other types of interneurons as well as excitatory principal neurons and possess a disinhibitory net effect in cortical circuits. In order to elucidate their targeting specificity, the output connectivity of VIP interneurons was studied at the subcellular level in barrel cortex of interneuron-specific Cre-driver mice, using pre- and postembedding electron microscopy. Systematically sampling VIP boutons across all layers, we found a substantial proportion of the innervated subcellular structures were dendrites (80%), with somata (13%), and spines (7%) being much less targeted. In layer VI, a high proportion of axosomatic synapses was found (39%). GABA-immunopositive ratio was quantified among the targets using statistically validated thresholds: only 37% of the dendrites, 7% of the spines, and 26% of the somata showed above-threshold immunogold labeling. For the main target structure "dendrite", a higher proportion of GABAergic subcellular profiles existed in deep than in superficial layers. In conclusion, VIP interneurons innervate non-GABAergic excitatory neurons and interneurons at their subcellular domains with layer-dependent specificity. This suggests a diverse output of VIP interneurons, which predicts multiple functionality in cortical circuitry beyond disinhibition.de
dc.language.isoengde
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectGABA; cortical circuit; electron microscopy; immunogold labeling; vasoactive intestinal polypeptidede
dc.subject.ddc612
dc.titleSubcellular Targeting of VIP Boutons in Mouse Barrel Cortex is Layer-Dependent and not Restricted to Interneuronsde
dc.typejournalArticlede
dc.identifier.doi10.1093/cercor/bhx220
dc.type.versionpublishedVersionde
dc.relation.pISSN1047-3211
dc.relation.eISSN1460-2199
dc.bibliographicCitation.volume27de
dc.bibliographicCitation.issue11de
dc.bibliographicCitation.firstPage5353de
dc.bibliographicCitation.lastPage5368de
dc.type.subtypejournalArticle
dc.identifier.pmid28968722
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalCerebral Cortexde


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