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Quantitation of eumelanin and pheomelanin markers in diverse biological samples by HPLC-UV-MS following solid-phase extraction

dc.contributor.authorAffenzeller, Susanne
dc.contributor.authorFrauendorf, Holm
dc.contributor.authorLicha, Tobias
dc.contributor.authorJackson, Daniel J.
dc.contributor.authorWolkenstein, Klaus
dc.date.accessioned2019-11-05T11:24:15Z
dc.date.available2019-11-05T11:24:15Z
dc.date.issued2019de
dc.relation.ISSN1932-6203de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/16606
dc.description.abstractEumelanin and pheomelanin are well known and common pigments found in nature. However, their complex polymer structure and high thermostability complicate their direct chemical identification. A widely used analytical method is indirect determination using HPLC with UV detection of both types of melanin by their most abundant oxidation products: pyrrole-2,3-dicarboxylic acid (PDCA), pyrrole-2,3,5-tricarboxylic acid (PTCA), thiazole-4,5-dicarboxylic acid (TDCA), and thiazole-2,4,5-tricarboxylic acid (TTCA). An increasing interest in pigmentation in biological research led us to develop a highly sensitive and selective method to identify and quantify these melanin markers in diverse biological samples with complex matrices. By introducing solid-phase extraction (SPE, reversed-phase) following alkaline oxidation we could significantly decrease background signals while maintaining recoveries greater than 70%. Our HPLC-UV-MS method allows for confident peak identification via exact mass information in corresponding UV signals used for quantitation. In addition to synthetic melanin and Sepia officinalis ink as reference compounds eumelanin markers were detected in brown human hair and a brown bivalve shell (Mytilus edulis). Brown feathers from the common chicken (Gallus g. domesticus) yielded all four eumelanin and pheomelanin markers. The present method can be easily adapted for a wide range of future studies on biological samples with unknown melanin content.de
dc.description.sponsorshipOpen-Access-Publikationsfonds 2019
dc.language.isoengde
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMelanin; Oxidation; Percutaneous transluminal coronary angioplasty; Feathers; High performance liquid chromatography; Ultraviolet spectroscopy; Pigments; Chickensde
dc.subject.ddc550
dc.titleQuantitation of eumelanin and pheomelanin markers in diverse biological samples by HPLC-UV-MS following solid-phase extractionde
dc.typejournalArticlede
dc.identifier.doi10.1371/journal.pone.0223552
dc.identifier.doi10.1371/journal.pone.0223552.g001
dc.identifier.doi10.1371/journal.pone.0223552.g002
dc.identifier.doi10.1371/journal.pone.0223552.g003
dc.identifier.doi10.1371/journal.pone.0223552.g004
dc.identifier.doi10.1371/journal.pone.0223552.t001
dc.identifier.doi10.1371/journal.pone.0223552.t002
dc.identifier.doi10.1371/journal.pone.0223552.t003
dc.identifier.doi10.1371/journal.pone.0223552.t004
dc.type.versionpublishedVersionde
dc.relation.eISSN1932-6203
dc.bibliographicCitation.volume14de
dc.bibliographicCitation.issue10de
dc.type.subtypejournalArticle
dc.identifier.pmid31622353
dc.bibliographicCitation.articlenumbere0223552de
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalPLOS ONEde


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