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Integrating DNA Barcoding and Traditional Taxonomy for the Identification of Dipterocarps in Remnant Lowland Forests of Sumatra

dc.contributor.authorCarneiro de Melo Moura, Carina
dc.contributor.authorBrambach, Fabian
dc.contributor.authorJair Hernandez Bado, Kevin
dc.contributor.authorKrutovsky, Konstantin V.
dc.contributor.authorKreft, Holger
dc.contributor.authorTjitrosoedirdjo, Sri Sudarmiyati
dc.contributor.authorSiregar, Iskandar Z.
dc.contributor.authorGailing, Oliver
dc.date.accessioned2019-11-04T14:49:24Z
dc.date.available2019-11-04T14:49:24Z
dc.date.issued2019de
dc.relation.ISSN2223-7747de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/16593
dc.description.abstractDNA barcoding has been used as a universal tool for phylogenetic inferences and diversity assessments, especially in poorly studied species and regions. The aim of this study was to contrast morphological taxonomy and DNA barcoding, using the three frequently used markers matK, rbcL, and trnL-F, to assess the efficiency of DNA barcoding in the identification of dipterocarps in Sumatra, Indonesia. The chloroplast gene matK was the most polymorphic among these three markers with an average interspecific genetic distance of 0.020. The results of the molecular data were mostly in agreement with the morphological identification for the clades of Anthoshorea, Hopea, Richetia, Parashorea, and Anisoptera, nonetheless these markers were inefficient to resolve the relationships within the Rubroshorea group. The maximum likelihood and Bayesian inference phylogenies identified Shorea as a paraphyletic genus, Anthoshorea appeared as sister to Hopea, and Richetia was sister to Parashorea. A better discriminatory power among dipterocarp species provided by matK and observed in our study suggests that this marker has a higher evolutionary rate than the other two markers tested. However, a combination of several different barcoding markers is essential for reliable identification of the species at a lower taxonomic levde
dc.language.isoengde
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectDipterocarpoideae; genetic distance; matK; rbcL; reference DNA library; trnL-F; tropical tree diversityde
dc.subject.ddc570
dc.titleIntegrating DNA Barcoding and Traditional Taxonomy for the Identification of Dipterocarps in Remnant Lowland Forests of Sumatrade
dc.typejournalArticlede
dc.identifier.doi10.3390/plants8110461
dc.type.versionpublishedVersionde
dc.relation.eISSN2223-7747
dc.bibliographicCitation.volume8de
dc.bibliographicCitation.issue11de
dc.type.subtypejournalArticle
dc.identifier.pmid31671512
dc.bibliographicCitation.articlenumber461de
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalPlantsde


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