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Blue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) Formation

dc.contributor.authorNeubert, Elsa
dc.contributor.authorBach, Katharina Marie
dc.contributor.authorBusse, Julia
dc.contributor.authorBogeski, Ivan
dc.contributor.authorSchön, Michael P.
dc.contributor.authorKruss, Sebastian
dc.contributor.authorErpenbeck, Luise
dc.date.accessioned2019-10-28T14:40:53Z
dc.date.available2019-10-28T14:40:53Z
dc.date.issued2019de
dc.relation.ISSN1664-3224de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/16550
dc.description.abstractNeutrophil Extracellular Traps (NETs) are produced by neutrophilic granulocytes and consist of decondensed chromatin decorated with antimicrobial peptides. They defend the organism against intruders and are released upon various stimuli including pathogens, mediators of inflammation, or chemical triggers. NET formation is also involved in inflammatory, cardiovascular, malignant diseases, and autoimmune disorders like rheumatoid arthritis, psoriasis, or systemic lupus erythematosus (SLE). In many autoimmune diseases like SLE or dermatomyositis, light of the ultraviolet-visible (UV-VIS) spectrum is well-known to trigger and aggravate disease severity. However, the underlying connection between NET formation, light exposure, and disease exacerbation remains elusive. We studied the effect of UVA (375 nm), blue (470 nm) and green (565 nm) light on NETosis in human neutrophils ex vivo. Our results show a dose- and wavelength-dependent induction of NETosis. Light-induced NETosis depended on the generation of extracellular reactive oxygen species (ROS) induced by riboflavin excitation and its subsequent reaction with tryptophan. The light-induced NETosis required both neutrophil elastase (NE) as well as myeloperoxidase (MPO) activation and induced histone citrullination. These findings suggest that NET formation as a response to light could be the hitherto missing link between elevated susceptibility to NET formation in autoimmune patients and photosensitivity for example in SLE and dermatomyositis patients. This novel connection could provide a clue for a deeper understanding of light-sensitive diseases in general and for the development of new pharmacological strategies to avoid disease exacerbation upon light exposure.de
dc.description.sponsorshipOpen-Access-Publikationsfonds 2019
dc.language.isoengde
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectneutrophilic granulocytes; NET formation; UV-Vis light; inflammation; ROS formation; riboflavinde
dc.subject.ddc610
dc.titleBlue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) Formationde
dc.typejournalArticlede
dc.identifier.doi10.3389/fimmu.2019.02428
dc.identifier.doi10.3389/fimmu.2019.02428.s001
dc.identifier.doi10.3389/fimmu.2019.02428.s002
dc.type.versionpublishedVersionde
dc.relation.eISSN1664-3224
dc.bibliographicCitation.volume10de
dc.type.subtypejournalArticle
dc.bibliographicCitation.articlenumber2428de
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalFrontiers in Immunologyde


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