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SRpHi ratiometric pH biosensors for super-resolution microscopy

dc.contributor.authorRichardson, Douglas S.
dc.contributor.authorGregor, Carola
dc.contributor.authorWinter, Franziska R.
dc.contributor.authorUrban, Nicolai T.
dc.contributor.authorSahl, Steffen J.
dc.contributor.authorWillig, Katrin I.
dc.contributor.authorHell, Stefan W.
dc.date.accessioned2019-10-14T12:21:00Z
dc.date.available2019-10-14T12:21:00Z
dc.date.issued2017de
dc.relation.ISSN2041-1723de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/16496
dc.description.abstractFluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy.Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to endosomes.de
dc.language.isoengde
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectbiosensors; fluorescence microscopyde
dc.subject.ddc573
dc.subject.ddc612
dc.titleSRpHi ratiometric pH biosensors for super-resolution microscopyde
dc.typejournalArticlede
dc.identifier.doi10.1038/s41467-017-00606-4
dc.type.versionpublishedVersionde
dc.relation.eISSN2041-1723
dc.bibliographicCitation.volume8de
dc.bibliographicCitation.issue1de
dc.type.subtypejournalArticle
dc.identifier.pmid28924139
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalNature Communicationsde


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