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Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry

dc.contributor.authorKabatas, Selda
dc.contributor.authorAgüi-Gonzalez, Paola
dc.contributor.authorHinrichs, Rena
dc.contributor.authorJähne, Sebastian
dc.contributor.authorOpazo, Felipe
dc.contributor.authorDiederichsen, Ulf
dc.contributor.authorRizzoli, Silvio O.
dc.contributor.authorPhan, Nhu T. N.
dc.date.accessioned2019-10-01T08:42:28Z
dc.date.available2019-10-01T08:42:28Z
dc.date.issued2019de
dc.relation.ISSN1364-5544de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/16452
dc.description.abstractMolecular imaging of targeted large biomolecules has been restricted in SIMS due to the limited number of probes containing SIMS-detectable isotopes. We introduce here new 19F-containing molecules that can be conjugated in a site-specific manner to nanobodies able to recognize fluorescent proteins (FPs) or mouse immunoglobulins (Igs). In this work, we demonstrate that it is possible to use the 19F-nanobodies to reveal the location of several cellular proteins previously tagged with FPs or Igs. This enables specific bio-imaging in SIMS for a vast repertoire of biomolecules, offering new opportunities to study specific structural and functional molecular interactions in biological specimens.de
dc.language.isoengde
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/614765/EU//NEUROMOLANATOMYde
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subjectmass spectrometry; molecular imaging; nanobodiesde
dc.subject.ddc610
dc.titleFluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometryde
dc.typejournalArticlede
dc.identifier.doi10.1039/C9JA00117D
dc.type.versionpublishedVersionde
dc.relation.pISSN0267-9477
dc.relation.eISSN1364-5544
dc.bibliographicCitation.volume34de
dc.bibliographicCitation.issue6de
dc.bibliographicCitation.firstPage1083de
dc.bibliographicCitation.lastPage1087de
dc.type.subtypejournalArticle
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalJournal of Analytical Atomic Spectrometryde


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