Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry
Kabatas, Selda ; Agüi-Gonzalez, Paola ; Hinrichs, Rena ; Jähne, Sebastian ; Opazo, Felipe ; Diederichsen, Ulf ; Rizzoli, Silvio O. ; Phan, Nhu T. N.
Citable Link (URL):http://resolver.sub.uni-goettingen.de/purl?gs-1/16452
Molecular imaging of targeted large biomolecules has been restricted in SIMS due to the limited number of probes containing SIMS-detectable isotopes. We introduce here new 19F-containing molecules that can be conjugated in a site-specific manner to nanobodies able to recognize fluorescent proteins (FPs) or mouse immunoglobulins (Igs). In this work, we demonstrate that it is possible to use the 19F-nanobodies to reveal the location of several cellular proteins previously tagged with FPs or Igs. This enables specific bio-imaging in SIMS for a vast repertoire of biomolecules, offering new opportunities to study specific structural and functional molecular interactions in biological specimens.
Sponsored by EU