dc.contributor.author | Egelkamp, Richard | |
dc.contributor.author | Zimmermann, Till | |
dc.contributor.author | Schneider, Dominik | |
dc.contributor.author | Hertel, Robert | |
dc.contributor.author | Daniel, Rolf | |
dc.date.accessioned | 2019-07-08T12:08:19Z | |
dc.date.available | 2019-07-08T12:08:19Z | |
dc.date.issued | 2019 | de |
dc.identifier.uri | http://resolver.sub.uni-goettingen.de/purl?gs-1/16254 | |
dc.description.abstract | Nitriles are organic molecules with –C≡N as functional group and often toxic for
living organisms. Detoxification can occur via nitrilases that degrade nitriles directly to
carboxylic acids and ammonia, or with nitrile hydratases and amidases that convert
nitriles to amides and subsequently to carboxylic acids and ammonia. Despite the
knowledge of enzymatic degradation pathways, the influence of these compounds on the
composition of bacterial communities is unknown. The tolerances of four phylogenetically
different bacterial strains without known nitrile detoxification systems (Agrobacterium
tumefaciens, Bacillus subtilis, Corynebacterium glutamicum, and Escherichia coli)
to the toxic effects of nine nitriles and the corresponding carboxylic acids were
determined. Based on these results, the effect of nitriles on diversity and composition
of compost-derived bacterial communities was monitored over time by 16S rRNA gene
amplicon-based andmetagenome analyses. Acetone cyanohydrin, 2-phenylpropionitrile,
and pyruvonitrile exhibited a lethal, phenylacetonitrile, 4-hydroxybenzonitrile, and
cyclohexanecarbonitrile a growth-suppressing and succinonitrile, acetonitrile, and
crotononitrile a growth-promoting effect on the studied communities. Furthermore,
each nitrile had a specific community-shaping effect, e.g., communities showing
growth-suppression exhibited high relative abundance of Paenibacillus. In general,
analysis of all data indicated a higher resistance of Gram-positive than Gram-negative
bacterial community members and test organisms to growth-suppressing nitriles. More
than 70 putative nitrilase-encoding and over 20 potential nitrile hydratase-encoding
genes were identified during analysis of metagenomes derived from nitrile-enrichments,
underlining the high yet often unexplored abundance of nitrile-degrading enzymes. | de |
dc.description.sponsorship | Open-Access-Publikationsfonds 2019 | |
dc.language.iso | eng | de |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | nitriles; nitrilases; nitrile hydratases; nitrile toxicity; nitrile degradation; metagenomics | de |
dc.subject.ddc | 570 | |
dc.title | Impact of Nitriles on Bacterial Communities | de |
dc.type | journalArticle | de |
dc.identifier.doi | 10.3389/fenvs.2019.00103 | |
dc.identifier.doi | 10.3389/fenvs.2019.00103.s001 | |
dc.identifier.doi | 10.3389/fenvs.2019.00103.s002 | |
dc.identifier.doi | 10.3389/fenvs.2019.00103.s003 | |
dc.identifier.doi | 10.3389/fenvs.2019.00103.s004 | |
dc.type.version | publishedVersion | de |
dc.relation.eISSN | 2296-665X | |
dc.bibliographicCitation.volume | 7 | de |
dc.type.subtype | journalArticle | |
dc.bibliographicCitation.articlenumber | 103 | de |
dc.description.status | peerReviewed | de |
dc.bibliographicCitation.journal | Frontiers in Environmental Science | de |