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COP9 Signalosome Interaction with UspA/Usp15 Deubiquitinase Controls VeA-Mediated Fungal Multicellular Development

dc.contributor.authorMeister, Cindy
dc.contributor.authorThieme; Karl G.
dc.contributor.authorThieme, Sabine
dc.contributor.authorKöhler, Anna M.
dc.contributor.authorSchmitt, Kerstin
dc.contributor.authorValerius, Oliver
dc.contributor.authorBraus, Gerhard H.
dc.date.accessioned2019-07-08T12:01:03Z
dc.date.available2019-07-08T12:01:03Z
dc.date.issued2019de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/16253
dc.description.abstractCOP9 signalosome (CSN) and Den1/A deneddylases physically interact and promote multicellular development in fungi. CSN recognizes Skp1/cullin-1/Fbx E3 cullin-RING ligases (CRLs) without substrate and removes their posttranslational Nedd8 modification from the cullin scaffold. This results in CRL complex disassembly and allows Skp1 adaptor/Fbx receptor exchange for altered substrate specificity. We characterized the novel ubiquitin-specific protease UspA of the mold Aspergillus nidulans, which corresponds to CSN-associated human Usp15 and interacts with six CSN subunits. UspA reduces amounts of ubiquitinated proteins during fungal development, and the uspA gene expression is repressed by an intact CSN. UspA is localized in proximity to nuclei and recruits proteins related to nuclear transport and transcriptional processing, suggesting functions in nuclear entry control. UspA accelerates the formation of asexual conidiospores, sexual development, and supports the repression of secondary metabolite clusters as the derivative of benzaldehyde (dba) genes. UspA reduces protein levels of the fungal NF-kappa B-like velvet domain protein VeA, which coordinates differentiation and secondary metabolism. VeA stability depends on the Fbx23 receptor, which is required for light controlled development. Our data suggest that the interplay between CSN deneddylase, UspA deubiquitinase, and SCF-Fbx23 ensures accurate levels of VeA to support fungal development and an appropriate secondary metabolism.de
dc.description.sponsorshipOpen-Access-Publikationsfonds 2019
dc.language.isoengde
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCOP9 signalosome (CSN), Aspergillus nidulans; Fbx23; Usp15/UspA; deubiquitination; fungal development; secondary metabolism; ubiquitin; ubiquitin-specific protease; velvet domain proteinsde
dc.subject.ddc572
dc.titleCOP9 Signalosome Interaction with UspA/Usp15 Deubiquitinase Controls VeA-Mediated Fungal Multicellular Developmentde
dc.typejournalArticlede
dc.identifier.doi10.3390/biom9060238
dc.type.versionpublishedVersionde
dc.relation.eISSN2218-273X
dc.bibliographicCitation.volume9de
dc.bibliographicCitation.issue6de
dc.type.subtypejournalArticle
dc.identifier.pmid31216760
dc.bibliographicCitation.articlenumber238de
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalBiomoleculesde


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