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Signal peptide peptidase activity connects the unfolded protein response to plant defense suppression by Ustilago maydis

dc.contributor.authorPinter, Niko
dc.contributor.authorHach, Christina Andrea
dc.contributor.authorHampel, Martin
dc.contributor.authorRekhter, Dmitrij
dc.contributor.authorZienkiewicz, Krzysztof
dc.contributor.authorFeussner, Ivo
dc.contributor.authorPoehlein, Anja
dc.contributor.authorDaniel, Rolf
dc.contributor.authorFinkernagel, Florian
dc.contributor.authorHeimel, Kai
dc.date.accessioned2019-05-08T09:53:04Z
dc.date.available2019-05-08T09:53:04Z
dc.date.issued2019de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/16094
dc.description.abstractThe corn smut fungus Ustilago maydis requires the unfolded protein response (UPR) to maintain homeostasis of the endoplasmic reticulum (ER) during the biotrophic interaction with its host plant Zea mays (maize). Crosstalk between the UPR and pathways controlling pathogenic development is mediated by protein-protein interactions between the UPR regulator Cib1 and the developmental regulator Clp1. Cib1/Clp1 complex formation results in mutual modification of the connected regulatory networks thereby aligning fungal proliferation in planta, efficient effector secretion with increased ER stress tolerance and long-term UPR activation in planta. Here we address UPR-dependent gene expression and its modulation by Clp1 using combinatorial RNAseq/ChIPseq analyses. We show that increased ER stress resistance is connected to Clp1-dependent alterations of Cib1 phosphorylation, protein stability and UPR gene expression. Importantly, we identify by deletion screening of UPR core genes the signal peptide peptidase Spp1 as a novel key factor that is required for establishing a compatible biotrophic interaction between U. maydis and its host plant maize. Spp1 is dispensable for ER stress resistance and vegetative growth but requires catalytic activity to interfere with the plant defense, revealing a novel virulence specific function for signal peptide peptidases in a biotrophic fungal/plant interaction.de
dc.description.sponsorshipOpen-Access-Publikationsfonds 2019
dc.language.isoengde
dc.rightsopenAccess
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectUstilago maydis; Gene expression; Plant fungal pathogens; Fungal genetics; Maize; Virulence factors; Plant defenses; Sequence motif analysisde
dc.subject.ddc572
dc.titleSignal peptide peptidase activity connects the unfolded protein response to plant defense suppression by Ustilago maydisde
dc.typejournalArticlede
dc.identifier.doi10.1371/journal.ppat.1007734
dc.identifier.doi10.1371/journal.ppat.1007734.g001
dc.identifier.doi10.1371/journal.ppat.1007734.g002
dc.identifier.doi10.1371/journal.ppat.1007734.g003
dc.identifier.doi10.1371/journal.ppat.1007734.g004
dc.identifier.doi10.1371/journal.ppat.1007734.g005
dc.identifier.doi10.1371/journal.ppat.1007734.g006
dc.identifier.doi10.1371/journal.ppat.1007734.g007
dc.identifier.doi10.1371/journal.ppat.1007734.g008
dc.identifier.doi10.1371/journal.ppat.1007734.g009
dc.identifier.doi10.1371/journal.ppat.1007734.s001
dc.identifier.doi10.1371/journal.ppat.1007734.s002
dc.identifier.doi10.1371/journal.ppat.1007734.s003
dc.identifier.doi10.1371/journal.ppat.1007734.s004
dc.identifier.doi10.1371/journal.ppat.1007734.s005
dc.identifier.doi10.1371/journal.ppat.1007734.s006
dc.identifier.doi10.1371/journal.ppat.1007734.s007
dc.identifier.doi10.1371/journal.ppat.1007734.s008
dc.identifier.doi10.1371/journal.ppat.1007734.s009
dc.identifier.doi10.1371/journal.ppat.1007734.s010
dc.identifier.doi10.1371/journal.ppat.1007734.s011
dc.identifier.doi10.1371/journal.ppat.1007734.s012
dc.identifier.doi10.1371/journal.ppat.1007734.s013
dc.identifier.doi10.1371/journal.ppat.1007734.s014
dc.identifier.doi10.1371/journal.ppat.1007734.s015
dc.identifier.doi10.1371/journal.ppat.1007734.s016
dc.identifier.doi10.1371/journal.ppat.1007734.s017
dc.identifier.doi10.1371/journal.ppat.1007734.s018
dc.identifier.doi10.1371/journal.ppat.1007734.s019
dc.identifier.doi10.1371/journal.ppat.1007734.s020
dc.identifier.doi10.1371/journal.ppat.1007734.s021
dc.identifier.doi10.1371/journal.ppat.1007734.s022
dc.identifier.doi10.1371/journal.ppat.1007734.s023
dc.type.versionpublishedVersionde
dc.relation.eISSN1553-7374
dc.bibliographicCitation.volume15de
dc.bibliographicCitation.issue4de
dc.type.subtypejournalArticle
dc.identifier.pmid30998787
dc.bibliographicCitation.articlenumbere1007734de
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalPLOS Pathogensde


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