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Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions.

dc.contributor.authorFornasiero, Eugenio F.
dc.contributor.authorMandad, Sunit
dc.contributor.authorWildhagen, Hanna
dc.contributor.authorAlevra, Mihai
dc.contributor.authorRammner, Burkhard
dc.contributor.authorKeihani, Sarva
dc.contributor.authorOpazo, Felipe
dc.contributor.authorUrban, Inga
dc.contributor.authorIschebeck, Till
dc.contributor.authorSakib, M. Sadman
dc.contributor.authorFard, Maryam K.
dc.contributor.authorKirli, Koray
dc.contributor.authorCenteno, Tonatiuh Pena
dc.contributor.authorVidal, Ramon O.
dc.contributor.authorRahman, Raza-Ur
dc.contributor.authorBenito, Eva
dc.contributor.authorFischer, André
dc.contributor.authorDennerlein, Sven
dc.contributor.authorRehling, Peter
dc.contributor.authorFeussner, Ivo
dc.contributor.authorBonn, Stefan
dc.contributor.authorSimons, Mikael
dc.contributor.authorUrlaub, Henning
dc.contributor.authorRizzoli, Silvio O.
dc.date.accessioned2018-11-13T13:38:37Z
dc.date.available2018-11-13T13:38:37Z
dc.date.issued2018de
dc.relation.ISSN2041-1723de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/15388
dc.description.abstractThe turnover of brain proteins is critical for organism survival, and its perturbations are linked to pathology. Nevertheless, protein lifetimes have been difficult to obtain in vivo. They are readily measured in vitro by feeding cells with isotopically labeled amino acids, followed by mass spectrometry analyses. In vivo proteins are generated from at least two sources: labeled amino acids from the diet, and non-labeled amino acids from the degradation of pre-existing proteins. This renders measurements difficult. Here we solved this problem rigorously with a workflow that combines mouse in vivo isotopic labeling, mass spectrometry, and mathematical modeling. We also established several independent approaches to test and validate the results. This enabled us to measure the accurate lifetimes of ~3500 brain proteins. The high precision of our data provided a large set of biologically significant observations, including pathway-, organelle-, organ-, or cell-specific effects, along with a comprehensive catalog of extremely long-lived proteins (ELLPs).de
dc.languageeng
dc.language.isoengde
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/339580/EU//MITRACde
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/614765/EU//NEUROMOLANATOMYde
dc.rightsopenAccess
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectprotein lifetimes; brain proteins;de
dc.subject.ddc573
dc.subject.ddc612
dc.titlePrecisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions.de
dc.typejournalArticlede
dc.identifier.doi10.1038/s41467-018-06519-0
dc.type.versionpublishedVersionde
dc.bibliographicCitation.volume9de
dc.bibliographicCitation.issue1de
dc.type.subtypejournalArticle
dc.identifier.pmid30315172
dc.bibliographicCitation.articlenumber4230de
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalNature Communicationsde


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