mRNA expression profiling in cotyledons reveals significant up-regulation of the two bovine pregnancy-associated glycoprotein genes boPAG-8 and boPAG-11 in early gestation
Citable Link (URL):http://resolver.sub.uni-goettingen.de/purl?gs-1/15375
Journal Article (Published version)
The multigene family of pregnancy-associated glycoproteins (PAGs) belongs to a group of aspartic proteases that are exclusively expressed by trophoblast cells in the placenta of even-toed ungulates. In Bovidae, 22 different PAG genes (boPAGs) with a wide range of temporal and spatial expression- and glycosylation patterns have been reported to date. In this study we describe the mRNA expression patterns using real-time quantitative PCR (qPCR) for selected modern (boPAG-1, -9, -21) and ancient bovine PAGs (boPAG-2, -8, -10, -11, - 12) in cotyledonary tissue. The highest mean expression was detected in boPAG-8 and lowest in boPAG-10 (P < 0.05). Furthermore, boPAG-8 and -11 were significantly greater expressed in early gestation compared with later pregnancy stages. The characterization of boPAG mRNA-expression levels gives important insights for further protein analyses which will be valuable information for the development of new pregnancy detection systems.