Show simple item record

Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa

dc.contributor.authorSiegler, Heike
dc.contributor.authorValerius, Oliver
dc.contributor.authorIschebeck, Till
dc.contributor.authorPopko, Jennifer
dc.contributor.authorTourasse, Nicolas J
dc.contributor.authorVallon, Olivier
dc.contributor.authorKhozin-Goldberg, Inna
dc.contributor.authorBraus, Gerhard H
dc.contributor.authorFeussner, Ivo
dc.description.abstractAbstract Background Lobosphaera incisa (L. incisa) is an oleaginous microalga that stores triacylglycerol (TAG) rich in arachidonic acid in lipid bodies (LBs). This organelle is gaining attention in algal research, since evidence is accumulating that proteins attached to its surface fulfill important functions in TAG storage and metabolism. Results Here, the composition of the LB proteome in L incisa was investigated by comparing different cell fractions in a semiquantitative proteomics approach. After applying stringent filters to the proteomics data in order to remove contaminating proteins from the list of possible LB proteins (LBPs), heterologous expression of candidate proteins in tobacco pollen tubes, allowed us to confirm 3 true LBPs: A member of the algal Major Lipid Droplet Protein family, a small protein of unknown function and a putative lipase. In addition, a TAG lipase that belongs to the SUGAR DEPENDENT 1 family of TAG lipases known from oilseed plants was identified. Its activity was verified by functional complementation of an Arabidopsis thaliana mutant lacking the major seed TAG lipases. Conclusions Here we describe 3 LBPs as well as a TAG lipase from the oleaginous microalga L. incisa and discuss their possible involvement in LB metabolism. This study highlights the importance of filtering LB proteome datasets and verifying the subcellular localization one by one, so that contaminating proteins can be recognized as such. Our dataset can serve as a valuable resource in the identification of additional LBPs, shedding more light on the intriguing roles of LBs in microalgae.
dc.publisherBioMed Central
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/266401/EU/Genetic Improvement of Algae for Value Added Products/GIAVAP
dc.identifier.bibliographicCitationBMC Plant Biology. 2017 Jun 06;17(1):98
dc.titleAnalysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa
dc.rights.holderThe Author(s).
dc.bibliographicCitation.journalBMC Plant Biology

Files in this item


This item appears in the following Collection(s)

Show simple item record

These documents are avalilable under the license: