dc.contributor.author | Martius, Gesa | |
dc.contributor.author | Cameron, Silke | |
dc.contributor.author | Rave-Fränk, Margret | |
dc.contributor.author | Hess, Clemens F. | |
dc.contributor.author | Wolff, Hendrik A. | |
dc.contributor.author | Malik, Ihtzaz A. | |
dc.date.accessioned | 2015-05-08T08:36:47Z | |
dc.date.available | 2015-05-08T08:36:47Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Martius, Gesa; Cameron, Silke; Rave-Fränk, Margret; Hess, Clemens F; Wolff, Hendrik A; Malik, Ihtzaz A (2015): The anti-TNF-α antibody infliximab inhibits the expression of fat-transporter-protein FAT/CD36 in a selective hepatic-radiation mouse model. - International journal of molecular sciences, Vol. 16, Nr. 3, p. 4682-97 | |
dc.relation.ISSN | 1422-0067 | |
dc.identifier.uri | http://resolver.sub.uni-goettingen.de/purl?gs-1/11783 | |
dc.description.abstract | Previously, we reported a radiation-induced inflammation triggering fat-accumulation through fatty-acid-translocase/cluster of differentiation protein 36 (FAT/CD36) in rat liver. Furthermore, inhibition of radiation-induced FAT/CD36-expression by anti-tumor necrosis factor-α (anti-TNF-α) (infliximab) was shown in vitro. The current study investigates fat-accumulation in a mouse-model of single-dose liver-irradiation (25-Gray) and the effect of anti-TNF-α-therapy on FAT/CD36 gene-expression. Mice livers were selectively irradiated in vivo in presence or absence of infliximab. Serum- and hepatic-triglycerides, mRNA, and protein were analyzed by colorimetric assays, RT-PCR, Immunofluorescence and Western-Blot, respectively. Sudan-staining was used demonstrating fat-accumulation in tissue. In mice livers, early (1-3 h) induction of TNF-α-expression, a pro-inflammatory cytokine, was observed. It was followed by elevated hepatic-triglyceride level (6-12 h), compared to sham-irradiated controls. In contrast, serum-triglyceride level was decreased at these time points. Similar to triglyceride level in mice livers, Sudan staining of liver cryosections showed a quick (6-12 h) increase of fat-droplets after irradiation. Furthermore, expression of fat-transporter-protein FAT/CD36 was increased at protein level caused by radiation or TNF-α. TNF-α-blockage by anti-TNF-α showed an early inhibition of radiation-induced FAT/CD36 expression in mice livers. Immunohistochemistry showed basolateral and cytoplasmic expression of FAT/CD36 in hepatocytes. Moreover, co-localization of FAT/CD36 was detected with α-smooth muscle actin (α-SMA+) cells and F4/80+ macrophages. In summary, hepatic-radiation triggers fat-accumulation in mice livers, involving acute-phase-processes. Accordingly, anti-TNF-α-therapy prevented early radiation-induced expression of FAT/CD36 in vivo. | |
dc.description.sponsorship | Open-Access-Publikationsfonds 2015 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.rights | openAccess | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | irradiation; fat accumulation; liver; FAT/CD36; TNF-α; infliximab | |
dc.title | The anti-TNF-α antibody infliximab inhibits the expression of fat-transporter-protein FAT/CD36 in a selective hepatic-radiation mouse model. | |
dc.type | journalArticle | |
dc.identifier.doi | 10.3390/ijms16034682 | |
dc.type.version | publishedVersion | |
dc.bibliographicCitation.volume | 16 | |
dc.bibliographicCitation.issue | 3 | |
dc.bibliographicCitation.firstPage | 4682 | |
dc.bibliographicCitation.lastPage | 4697 | |
dc.type.subtype | journalArticle | |
dc.identifier.pmid | 25739082 | |
dc.description.status | peerReviewed | |
dc.bibliographicCitation.journal | International journal of molecular sciences | |