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Grafting of Neonatal marmoset monkey testicular single-cell suspensions into immunodeficient mice leads to ex situ testicular cord neomorphogenesis.

dc.contributor.authorAeckerle, Nelia
dc.contributor.authorDressel, Ralf
dc.contributor.authorBehr, Rüdiger
dc.date.accessioned2014-09-08T08:54:04Z
dc.date.available2014-09-08T08:54:04Z
dc.date.issued2013
dc.identifier.citationAeckerle, Nelia; Dressel, Ralf; Behr, Rüdiger (2013): Grafting of Neonatal marmoset monkey testicular single-cell suspensions into immunodeficient mice leads to ex situ testicular cord neomorphogenesis. - Cells, tissues, organs, Vol. 198, Nr. 3, p. 209-20
dc.relation.ISSN1422-6421
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/10817
dc.description.abstractSingle-cell suspensions derived from immature rodent and ungulate testes can reconstitute testicular cords upon grafting into immunodeficient mice. In the present study, neonatal common marmoset monkey (Callithrix jacchus) testes were digested to a single-cell suspension, which was transplanted subcutaneously into immunodeficient mice. After 9 or 18 weeks of incubation, the derivatives of the grafted single-cell suspensions were retrieved and analyzed histologically and immunohistochemically. Three of 4 (75%) neonatal grafts exhibited reconstituted seminiferous cords strongly resembling seminiferous cords of the intact neonatal testis. The cords consisted of Sertoli cells, germ cells and peritubular myoid cells, which was confirmed by immunohistochemical marker analysis. Three-dimensional reconstruction models of the grafts revealed elongated tubules. Some of the tubules were branched, which occurs also in vivo, as we show here for the marmoset monkey. Importantly, no teratoma formation by immature pluripotency factor-expressing germ cells was observed. In summary, the reconstituted testicular cords were almost indistinguishable from the cords formed in situ, thereby impressively demonstrating a very high reconstructive potential of a single-cell suspension obtained from the neonatal marmoset monkey testis. To our knowledge, this is the first study demonstrating testicular cord neomorphogenesis for a primate species ex situ.
dc.languageeng
dc.language.isoeng
dc.rightsopenAccess
dc.subjectGerm cells; Non-human primate; Pluripotency; Sertoli cell; Testicular morphogenesis
dc.titleGrafting of Neonatal marmoset monkey testicular single-cell suspensions into immunodeficient mice leads to ex situ testicular cord neomorphogenesis.
dc.typejournalArticle
dc.identifier.doi10.1159/000355339
dc.type.versionpublishedVersion
dc.identifier.fs601935
dc.bibliographicCitation.volume198
dc.bibliographicCitation.issue3
dc.bibliographicCitation.firstPage209
dc.bibliographicCitation.lastPage220
dc.type.subtypejournalArticle
dc.identifier.pmid24192033
dc.notesThis publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
dc.description.statuspeerReviewed
dc.bibliographicCitation.journalCells, tissues, organs


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